Immunogen | B-lymphoma cells |
Product Type | Antibodies |
Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch. |
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Format | State: Liquid purified Ig |
? | Purification: Affinity chromatography on protein A |
? | Buffer System: 10mM PBS/1% BSA buffer pH 7.4 with 0.09% sodium azide |
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Specificity | This antibody reacts to Kappa-Light-Chain. |
Application | Immunohistochemistry on paraffin Sections: 1/200 for 30 minutes at RT; Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min; Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Positive Control: Tonsil |
Background | Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pentamer. IgA may also form polymers. Kappa light chain contains one immunoglobulin like domain. |