Immunogen | Purified mouse IgG. Spleen cells from immunised PVG RT1c rats were fused with cells of the rat Y3.Ag.1.2.3 myeloma cell line. |
Product Type | Antibodies |
Recommended Isotype Control | SM19B |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
References | 1. Galfre, G. et al. (1979) Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG Nature 277:131-133. |
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Format | State: Liquid purified IgG fraction. |
? | Label: Biotin |
? | Purification: Ion Exchange Chromatography. |
? | Buffer System: PBS, pH 7.4, containing 0.09% Sodium Azide as preservative and 1% Bovine Serum Albumin as stabilizer. |
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Specificity | This antibody recognises murine kappa immunoglobulin light chains. No reactivity is seen against mouse lambda light chains in ELISA assays. |
Application | Flow Cytometry: Use 10 ?l of 1/50-1/100 diluted antibody to label 10e6 cells in 100 ?l. |
Concentration | 1.0 mg/ml |