Synonyms | TSGA; JMJD1; KDM3A; JHDM2A; JHMD2A; KIAA0742; DKFZp686A24246; DKFZp686P07111; JMJD1A |
Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human JHDM2A. |
Product Type | Antibodies |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
References | 1. Wellmann,S., Biochem. Biophys. Res. Commun. 372 (4), 892-897 (2008) 2. Yamane,K., Cell 125 (3), 483-495 (2006) |
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Format | State: Liquid purified Ig fraction. |
? | Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
? | Buffer System: PBS containing 0.09% (W/V) Sodium Azide as preservative. |
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Specificity | This antibody is specific to JHDM2a (N-term). |
Application | ELISA: 1/1,000. Immunohistochemistry: 1/10-1/50. |
Background | JHDM2a is a zinc finger protein that contains a jumonji domain. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. This protein preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. It is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. |
Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Concentration | 0.25 mg/ml |