Synonyms | SERPINA1, AAT |
Swiss Prot Num | P01009 |
Immunogen | Human Alpha-1-Antitrypsin purified from plasma |
Product Type | Antibodies |
Storage | Store the antibody undiluted at 2-8 °C. Shelf life: one year from despatch. |
|
Format | State: Liquid purified Ig fraction |
? | Purification: Delipidated and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbants. An immunoglobulin fraction is produced by ion-exchange chromatography. |
? | Buffer System: Glycine buffered saline, pH 7.4, containing 0.09 % Sodium Azide, 0.1% E-Amino-n-Caproic Acid, 0.01% Benzamidine and 1mM EDTA as preservatives |
|
Specificity | Gives a single arc when tested by IEP against human plasma. Identity is confirmed by double diffusion (Ouchterlony) against human plasma and a known anti-human alpha-1-Antitrypsin. |
Application | Suitable for use in a variety of gel techniques including radial immunodiffusion (RID), double diffusion, and immunoelectrophoresis (IEP). The use of 3 % PEG 6000 with 1.2 % agarose in a suitable buffer (such as Tris borate EDTA or Tris-barbital pH >8.2) is recommended. RID: 2 ?l antiserum/cm2 gel vs 5 ?l 1:2 - 1:50 diluted human serum. Double Diffusion: 10 ?l antiserum vs 5 ?l serum. IEP: 100 ?l antiserum vs 5 ?l serum. |
Concentration | 24 mg/ml (OD280nm, E1% = 14.5) |