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| Cat. No |
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Description |
Unit |
Price
(VAT별도) |
납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-351808 |
NB120-12358 |
Agrin Antibody (Agr 33)//Mouse, Rat/Mouse |
1/EA |
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문의 |
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Subject
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Agrin Antibody (Agr 33) |
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Description
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/ Mouse, Rat/Mouse |
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Clonality
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Mono |
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Company
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Novus biologicals |
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Application
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ICC, Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Species: | Rt, Mu | | Applications: | WB, ICC | | Clonality: | Monoclonal | | | Gene: | AGRN | | Purity: | protein G purified | | Host: | Mouse | | | Specificity: | detects all isoforms of agrin. | | Publications: | Antibody has not yet been mentioned in a publication. | Details: | Clone: | Agr 33 | | Isotype: | IgG1 | | Immunogen: | Recombinant rat agrin, C-terminal construct. | | Epitope: | Epitope has been mapped ne ar the 2nd laminin doma in homology region and causes a marked reducti on in the number of nicoti nic acetylcholine receptor (nAChR) clusters. Inserti on of an 8aa peptide at sp licing site Z increases th e clustering activity | | Localization: | Synaptic basal lamina at the neuromuscular junction. | Gene Symbol: AGRN | | 11603 (Mouse) | | | 25592 (Rat) | | Related Genes: | ACHR, MUSK, DAG1, CD44, UTRN, RAPSN, DMD, HNRNPC, C2, ACHE, SDC2, AQP4, EGF, NRG1, MMP3, PRSS7, ERBB2, SYP, DG, CHRNA4, CHL1, NCAM1, FYN, MAP3K14, SPARC, MUC3A, NDUFA2, SRC, ITGB1, EIF3K, EGFR, AKT1, PTPN11, GPHN, C21orf63, MAPK1, FOS, PIK3CG, BDNF, ABL1 | Background: Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa. Species: | Reactivity: | Cross-reacts with Mouse and Rat.Not yet tested in other species. | Applications: | Uses: | ICC: Use at a concentration of 2 ug/ml. WB: Use at a concentration of 2 ug/ml. Predicted molecular weight: 208.6 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. | | Dilutions: | Immunocytochemistry, Western Blot | | Concentrations: | 1.0 mg/ml | Packaging: | Buffer: | PBS, pH 7.2, 50% glycerol | | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | | Preservative: | 0.09% Sodium Azide | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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