| Synonyms | T3/Leu-4 |
| Swiss Prot Num | P22646 |
| Immunogen | C6VL-BS cell lysate |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Protocols | FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyteu-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 ul of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0.5 ug* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4?°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.) 7. Wash 2 times at 4?°C. 8. Resuspend the cell pellet in 50 ul ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 ul of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. MEDIA A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 ul of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 ul of 2M sodium azide in 100 mls). Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: C57Bl/6 Cell Concentration: 1x10e6 cells per test Antibody Concentration Used: 0.5 ug/10e6 cells Isotypic Control: FITC Hamster IgG |
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| Format | State: Liquid purified IgG |
| ? | Label: FITC |
| ? | Buffer System: PBS, 0.1% NaN3 and EIA grade BSA as stabilizing protien to bring total protein concentration to 4-5 mg/ml |
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| Specificity | This anti-mouse CD3u monoclonal antibody is specific for the u chain of mouse CD31. Immobolized 500A2 mAb can be used to stimulate mouse T cells in vitro1. A combination of immobolized 500A2 mAb and anti-mouse CD28 mAb is commonly used for the activation of mouse T cells3. |
| Application | Flow Cytometry (see Protocols). (Reportet to be useful in immunoprecipitation, immunofluorescent staining and immunohistochemistry on acetone fixed frozen sections.) |
| Background | In the mouse, CD3 is highly expressed by mature T cells and at lower levels by CD4+CD8+ thymocytes2. The u chain of CD3 is a component of the multimeric T cell receptor complex and is involved with signal transduction |
| Concentration | 0.1 mg/ml |
