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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
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Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-24-66134 |
CL001 |
Hamster Anti-CD3e (T3) Monoclonal Antibody, Unconjugated, Clone 145-2C11/0.5 ml/Mouse/Hamster |
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Subject
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Hamster Anti-CD3e (T3) Monoclonal Antibody, Unconjugated, Clone 145-2C11 |
Description
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0.5 ml / Mouse/Hamster |
Clonality
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Mono |
Company
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Acris Antibodies GmbH |
Application
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Flow cytometry, Functional assay |
Conjugation
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Unconjugated |
Immunogen
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CD3e (T3) |
Contents
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Description: Manufacturer | Acris Antibodies GmbH | Quantity | 0.5 ml | Reactivity | Mouse | Presentation | Ascites | Applications | Flow cytometry, Functional assay | Isotype | IgG | Host | Hamster | Clone | 145-2C11 | PDF datasheet | view | Shipping to | Worldwide | Synonyms | T3/Leu-4 | Swiss Prot Num | P22646 | Immunogen | H-2Kb sp. Donor: Armenian Hamster Spleen. Fusion Partner: Murine myeloma cell line SP2/0. | Product Type | Antibodies | Storage | Prior to reconstitution store at 2-8°C to -20°C. Following reconstitution store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | References | 1. Leo, O., Foo. M., Sachs, D.H. Samelson, L.E. and J.A. Bluestone. 1987. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl Acad. Sci. USA 84: 1374 - 1378. 2. Portoles, P., Rojo, J., Golby, A., Bonneville, M,. Gromkowski, S., Greenbaum, L., Janeway, G., Murphy, D., and K. Bottomly. Monoclonal Antibodies to Murine CD3e Define Distinct Epitopes, One of which may interact with CD4 During T Cell Activation. J. of Immunology 142: 4168 - 4175. 1989. 3. Bluestone, J.A., Pardoll, D., Sharrow, S.O. and B.J. Fowlkes. 1987. Characterization of murine thymocytes with CD3 - associated T cell receptor structure. Nature. 326, 82 - 84. 4. Hirsch, R., Gress, R.E., Pluznik, D.H., Eckhaus, M. and J.A. Bluestone. 1989. Effects of in vitro Administration of Anti-CD3 Monoclonal Antibody on T Cell Function in Mice II. In Vitro Activation of T Cells. J. of Immunology 142: 737-743. 5. Hirsch, R., Eckhaus, M., Auchincloss, H., Jr., Sachs, D.H. and J.A. Bluestone. 1988. Effects of in vivo Administration of Anti-T3 Monoclonal Antibody on T Cell Function in Mice. J. of Immunology 140: 3766-3772. | |
Format | Reconstitution: Restore with 0.5 ml of cold distilled water. | ? | State: Lyophilized Ascites | Protocols | FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare cell suspension in Media A. For cell preparations, deplete the red blood cell population with LympholyteuM cell separation medium. 2. Wash 2 times. 3. Resuspend cells to 1x10e6 cells in approximately 50 ul Media A in a microcentrifuge tube (ie. 50 ul of cells resuspended to 2x10e7 cells/ml). THE CONTENTS OF 1 TUBE REPRESENTS 1 TEST). 4. To each tube add 50 ul of 1/250-1/500 dilution of CL001. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4?¡ÆC. (It is recommended that tubes are protected from light since most fluorochromes are light sensitive). 7. Wash 2 times at 4?¡ÆC. 8. Add 100 ul of secondary antibody (FITC Goat a hamster IgG;) at 1/70 dilution. PLEASE NOTE: Do not use PE Goat a hamster IgG as the secondary antibody. 9. Incubate tubes at 4?¡ÆC for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4?¡ÆC in Media B. 11. Resuspend the cell pellet in 50 ul ice cold Media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 ul of propidium iodide at 0.5 mg/ml in phosphate buffered saline. (This stains dead cells by intercalating DNA). Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide ( 100 ul of 2 M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% bovine serum albumin + sodium azide (100 ul of 2 M sodium azide in 100 mls). | |
Specificity | This anti-mouse CD3epsilon monoclonal antibody is specific for a 25 kDa protein component (epsilon-T3) of the antigen specific T cell receptor on all mouse strains tested. The epsilon-T3 protein has been shown to be non-covalently associated with the cell surface alpha-beta heterodimer of the CD3 associated complex. This monoclonal antibody reacts with all mature T cells and can both activate and inhibit T cell function (1). This fact identifies epsilon-T3 as a cell surface protein involved in the transduction of activation signals. All peripheral T cells express this determinant; however B cells and bone marrow cells have proven to be negative. Although the expression of this particular epitope on peripheral T cells is uniformly high, staining of thymocytes reveals distinct subpopulations of cells differing in the level of expression of this marker. This antibody is useful in studying the role of various components of the TCR complex in T cell activation and development. This antibody will allow for the development of an animal model in which to investigate the immunoregulatory effects of in vivo administration of anti-CD3 antibodies, an area of obvious clinical importance. In addition, this monoclonal antibody, clone 145-2C11 was specifically designed to trigger T cell activation. | Application | Flow Cytometry (see Protocols) | Concentration | 1/250 (1x10e6 cells) | |
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