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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-24-66137 |
CL002P |
Hamster Anti-CD3e (T3) Monoclonal Antibody, Unconjugated, Clone 500-A2/0.2 mg/Mouse/Hamster |
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Subject
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Hamster Anti-CD3e (T3) Monoclonal Antibody, Unconjugated, Clone 500-A2 |
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Description
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0.2 mg / Mouse/Hamster |
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Clonality
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Mono |
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Company
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Acris Antibodies GmbH |
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Application
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Frozen sections, Flow cytometry, Immunoprecipitation |
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Conjugation
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Unconjugated |
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Immunogen
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CD3e (T3) |
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Contents
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Description: | Manufacturer | Acris Antibodies GmbH | | Quantity | 0.2 mg | | Reactivity | Mouse | | Presentation | Purified | | Applications | Frozen sections, Flow cytometry, Immunoprecipitation | | Isotype | IgG | | Host | Hamster | | Clone | 500-A2 | | PDF datasheet | view | | Shipping to | Worldwide | | Synonyms | T3/Leu-4 | | Swiss Prot Num | P22646 | | Product Type | Antibodies | | Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | | References | 1. Allison, J.P., et al. 1988. The T-Cell Receptor, UCLA Symposia, 73rd Ed. pp. 33-45. 2. Havran, W.L, et al. 1987. Nature. 330: 170-173 3. Harding, F.A., et al. 1992. Nature. 356: 607-609 4. Havran, W. L. et al. 1989. Proc. Nat. Acad. Sci. 86: 4185-4189. 5. Gross, J.A. et al. 1992. Nature. 149: 380-388. | | Protocols | FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyteu-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 ul of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1.0 ug* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4?°C. 7. Wash 2 times at 4?°C. 8. Add 100 ul of secondary antibody (FITC anti-hamster IgG) at a 1:70 dilution. 9. Incubate tubes at 4?°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4?°C. 11. Resuspend the cell pellet in 50 ul ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 ul of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. MEDIA: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 ul of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 ul of 2M sodium azide in 100 mls). | |
| Format | State: Liquid purified IgG | | ? | Buffer System: PBS, 0.09% NaN3 | |
| Specificity | This anti-mouse CD3u monoclonal antibody is specific for the uu?chain of mouse CD31. Immobolized 500A2 mAb can be used to stimulate mouse T cells in vitro1. A combination of immobilized 500A2 mAb and anti-mouse CD28 mAb is commonly used for the activation of mouse T cells3. | | Application | Flow cytometry (see Protocols). | | Background | In the mouse, CD3 is highly expressed by mature T cells and at lower levels by CD4+CD8+ thymocytes2. The u chain of CD3 is a component of the multimeric T cell receptor complex and is involved with signal transduction. | | Concentration | 0.2 mg/ml | |
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