| Synonyms | Integrin alpha-1, ITGA1, VLA-1 |
| Swiss Prot Num | Q3V3R4 |
| Immunogen | C1300 mouse neuroblastoma cell line. Spleen cells from immunised Armenian Hamsters were fused with cells of the mouse P3U1 mouse myeloma cell line. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Miyake, S. et al. (1994) Identification of collagen and laminin receptor integrins on murine T lymphocytes. Eur. J. Immunol. 24: 2000-2005. |
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| Format | State: Liquid purified IgG fraction. |
| ? | Label: Fluorescein Isothiocyanate Isomer 1 (FITC). |
| ? | Purification: Affinity Chromatography on Protein G. |
| ? | Buffer System: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. |
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| Specificity | This antibody recognises alpha 1 integrin (CD49a). Clone HM alpha 1 is reported to block VLA-1 dependent adhesion. We recommend the use of SM2099LE for this purpose. |
| Application | Flow Cytometry: (Neat -1/5): Use 10?l of the suggested working dilution to label 10e6 cells in 100 ?l. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. |
| Background | CD49a is a 180kD transmembrane glycoprotein, which associates with the beta 1 intergrin subunit to form the CD49a/CD29 (VLA-1) heterodimer. CD49a is expressed on activated T lymphocytes and at low levels on monocytes. VLA-1 is a receptor for laminin and collagen and plays an important role in regulating adhesion. |
| Concentration | 0.1 mg/ml |
