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AptaHotTaq is highly suited for qPCR applications. AptaHotTaq minimizes primer-dimer formation, reduces unspecific oligonucleotide priming and therefore, enhances target-specific amplification. AptaHotTaq, which possesses a 5´→ 3´ polymerase activity and a 5´ flap endonuclease activity is a mixture of the thermostable Taq DNA Polymerase with DNA-aptameres inhibiting the enzyme's activity at temperatures up to 50 °C. At temperatures above 50 °C the DNA-aptameres reversibly dissociate from the enzyme. The polymerase is activated during normal cycling conditions, allowing for a convenient assembly of PCR reactions at room-temperature. AptaHotTaq can be used in typical Taq-based cycling protocols. A separate high temperature incubation step to activate the enzyme is not needed but may help to resolve secondary structures of template DNA that may interfere with the initial amplification cycles.
■ Bioron |
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