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SSB-HotTaq is highly suited for hot-start PCR, qPCR and regular PCR applications. SSB-HotTaq minimizes primer/dimer formation, reduces unspecific oligonucleotide priming and, therefore, enhances target-specific amplification. SSB-HotTaq, which possesses a 5´→ 3´ polymerase activity and a 5´ flap endonuclease activity is a mixture of the thermostable Taq DNA Polymerase with a single-strand DNA binding (SSB) protein. The SSB protein binds to the PCR primers, preventing unspecific priming and amplification activity at lower temperatures. This allows for a convenient setup of PCR reactions at room-temperature, which also facilitates binding of the SSB protein to the oligonucleotides. The SSB protein becomes denatured during the initial denaturation step when amplification reactions are heated to 94 – 95 °C and bound oligonucleotides are now released for specific target priming. This allows for hot-start PCR in which the DNA polymerase finds an initiation complex for extension only after the first primer annealing step. SSB-HotTaq can be used in typical Taq-based cycling protocols.
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