The RT-PCR kit is designed especially for simple and effective one-tube RT-PCR. The reverse transcription step is directed by M-MuLV Reverse Transcriptase, as soon as cDNA is synthesized, a chemically modified “Hot-Start” Taq DNA polymerase, is activated by heating up the reaction 10 min at 95 °C. As soon as the Taq-star enzyme is activated, regular PCR procedure is performed. An optimized reaction buffer provides good performance both for Reverse Transcriptase and for Taq DNA Polymerase. The buffer qA provides good performance in TaqMan assay and in SYBR Green Real Time analysis as well.