Ron’s family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety of sources. Key of the system is the HiBind matrix that reversibly binds DNA or RNA under certain conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer.
HiBind mini-columns facilitate the binding, washing, and elution steps thus enabling multiple samples to be simultaneously processed. Yields vary according to plasmid copy number, E. coli strain and conditions of growth, but 5 ml of overnight culture with high copy-number plasmids in LB medium typically produce 15-25 µg plasmid DNA. For more DNA, we recommend the Ron’s Plasmid Midi Kit that yields 40-75 µg DNA from 10-15 ml culture when using high copy-number plasmids.

Plasmid-DNA isolated with the Ron’s Plasmid Mini Kit is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, and other applications.

Ron’s Plasmid Mini Kits are available with Classic-Line columns which do not have lids for a more comfortable handling.

The Ron’s Plasmid Mini Kits combine the power of HBind technology with the consistency of alkaline-SDS lysis of bacterial cells to deliver high quality DNA. Plasmid DNA binds effectively to the silica membrane of the HBind column and can easily be purified from contaminants and enzyme inhibitors. Purified plasmid DNA is simply eluted from the column in Elution buffer or water.
Ron’s Plasmid Mini Kits are available with Classic-Line colums which do not have lids for a more comfortable handling.

Miniprep Kit: 25 μg plasmid DNA
Midiprep Kit: 100 μg plasmid DNA
Maxiprep Kit: 500 μg plasmid DNA