Principle
The PCR reaction volume is determined, an equal amount of Binding Buffer XP1 was added to the sample and then applied to a HiBind DNA spin-column. DNA will reversibly bind to the silica matrix and, following a rapid washing step, where salts, free nucleotides, oligonucleotides and polymerases are removed, the DNA is eluted with deionized water, elution buffer or low salt buffer and is ready for further applications.
