The Ron’s family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety of sources. Key of the system is the HiBind matrix that specifically, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer.
Gel purification of DNA is a common technique for isolation of specific fragments from reaction mixtures. However, most methods either fail to completely remove agarose (which can lead to problems in downstream applications), shear the DNA, or result in very low yields. The Ron’s Gel Extraction Kit uses HiBind technology to recover DNA bands between 50 bp and 40 kb from all grades of agarose gel in yields reaching 90 – 95 %. Each HiBind DNA column can bind up to 30 µg DNA.
The DNA band of interest is excised from the gel, dissolved in Binding Buffer, and applied to a HiBind DNA spin-column. Following a rapid washing step, DNA is eluted with deionized water (or low salt buffer) and ready for further applications. The purified DNA is suitable for ligations, PCR sequencing, restriction digestion, or various labeling reactions.
