Principle
Ron¡¯s Tissue DNA Mini Kits use the reversible binding properties of the HiBind matrix, a silica-based material, combined with the speed of mini-column spin technology. A specifically formulated buffer system allows up to 30 µg of genomic DNA with fragment sizes up to 60 kb to bind to the matrix.
Samples are first homogenized, lysed under denaturing conditions and then applied to the HiBind spin columns, where the DNA is effectively bound to the silica membrane. Cellular debris, proteins and other contaminants are washed away by specific buffers. The high quality DNA is finally eluted in Elution buffer or sterile deionized water.