Fragment sizes (Base Pairs)
8454, 7242, 6369, 5686, 4822, 4324, 3675, 2323, 1929, 1371, 1264, 702, 224, 117
Attention!
The cohesive ends of fragments 1 and 4 may cause formation of extra band 14,140 bp. The fragments can be separated by heating to 65¡ÆC for 3 minutes before loading the sample on the gel.