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Loading Buffer II (6x)   

The loading dye increases the density of the sample and they add colour to the sample, thereby simplifying the loading process. The solution contains dyes that, in a electric field, move toward the anode at predictable rates.

In 1% agarose gels, bromophenol blue migrates with 300 bp linear double-stranded DNA fragment, whereas xylene cyanol FF migrates at approximately the same rate as linear double-stranded DNA 4 kb length. These relationships are not significantly affected by the concentration (0.5 to 1.4%) of agarose in the gel.

The gel – loading buffer contains only one low concentration dye (bromophenol blue and xylene cyanol FF) to avoid masking the DNA Ladder fragments. But if the added dye is masking your signal because it is running on the same high in your gel, just dilute it more.

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Loading Buffer II (6x)
Cat. No Model Description Unit Price
(VATº°µµ)
³³±â Àç°í Catalog ÁÖ¹®/°ßÀû
LC-176-0110 306105  Loading Buffer IV (10x) 0.5 ml 1/EA 4,810¿ø 15 Day 0  
LC-176-0111 306105L  Loading Buffer IV (10x) 10 x 0.5 ml 1/EA 44,690¿ø 15 Day 0