Avarage fragments size – 50-300 bp;
A260/A280: 1.78;
amount of genomic (non-repetitive DNA) – 2%.
The sample as well as original material were tested for the absence of HIV1,2 RNA, HCV RNA and HBV DNA.
Applications
COT I Human DNA can be used for suppressing crosshybridization to human repetitive DNA in filter and microarray hybridizations and in situ hybridization experiments. Repetitive elements present in a probe may result in unspecific hybridization signals. To enable specific hybridization of the probe to the chromosomal target site the probe is denatured together with an excess of unlabeled COT I Human DNA as a competitor. As a result single-cope gene hybridization is not hindered by repetitive sequences.
