Enzymatic indirect non-radioactive labeling of DNA in PCR, nick-translation, cDNA synthesis or primer extension reactions.
Can be incorporated into DNA using reverse transcriptases, Taq DNA Polymerase, Klenow Fragment.
The resulting aminoallyl-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten, resulting in a uniform and high density labeling of DNA.
