7-deaza-dGTP may be effectively incorporated into the DNA by DNA polymerases and/or terminal deoxynucleotidyltransferase. 7-deaza-dG – containing DNA duplexes demonstrate increased stability, 7-deaza-dG – containing DNA penetrates cell membrane more effectively due to the increased lypophility.
7-deaza dGTP may be used in sequencing for the resolution of the compressions in G-C-rich regions. Amplification of some DNA regions is often hindered due to the high G-C content. Replacement of dGTP to 7-deaza dGTP in the master mix may solve the problems.